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MADISON, Wis. (AP) — A coalition of conservation groups filed a last-minute federal lawsuit seeking to stop plans to build the high-voltage Hickory-Cardinal transmission line across a Mississippi River wildlife refuge.

American Transmission Company, ITC Midwest and Dairyland Power Cooperative Inc. want to build a 102-mile (164-kilometer), 345-kilovolt line linking Iowa’s Dubuque County and Wisconsin’s Dane County. The cost of the line is expected to top half a billion dollars but the utilities contend the project would improve electrical reliability across the region.

A portion of the line would run through the Upper Mississippi River National Wildlife and Fish Refuge near Cassville, Wisconsin. The federal wildlife refuge is a haven for fish, wildlife and migratory birds that use it as their breeding grounds within the Mississippi Flyway. Millions of birds fly through the refuge, and it’s the only stopping point left for many migratory birds.

Opponents have been working to stop the project for years. The National Wildlife Federation, the Driftless Area Land Conservancy and the Wisconsin Wildlife Federation filed an action in federal court in Madison on Wednesday seeking an injunction to block the refuge crossing.

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The groups argue that the U.S. Fish And Wildlife Service issued final approvals for the refuge crossing in February without giving the public a chance to comment.

They also contend that the FWS and the utilities improperly reached a deal calling for the utilities to transfer about 36 acres (15 hectares) south of Cassville into the refuge in exchange for 19 acres (8 hectares) within the refuge for the line. The groups argue the deal violates the 1997 National Wildlife Refuge System Improvement Act, which establishes a formal process for determining refuge use.

The groups went on to argue in their filing that they need an injunction quickly because the utilities are already creating construction staging areas on both the Iowa and Wisconsin sides of the river to begin work on the crossing.

The lawsuits names the FWS, the refuge’s manager and the U.S. Army Corps of Engineers as defendants. Online court records showed U.S. Department of Justice attorney Kimberly Anne Cullen is representing them. She referred questions to U.S. DOJ spokesperson Matthew Nies, who didn’t immediately respond to an email message.

Media officials for American Transmission Company and Dairyland Power Cooperative had no immediate comment. No one immediately responded to an email message left in ITC Midwest’s general media inbox.